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human gal 9 protein  (R&D Systems)


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    Structured Review

    R&D Systems human gal 9 protein
    Human Gal 9 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+gal+9+protein/Recombinant+Human+Galectin-9+Protein%2C+CF/bio_rxiv__2025__07__31__666261-48-17-20
    Average 93 stars, based on 31 article reviews
    human gal 9 protein - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Early Steps of Individual Multireceptor Viral Interactions Dissected by High-Density, Multicolor Quantum Dot Mapping in Living Cells
    Article Snippet: Monoclonal mouse antihuman CD44 (Clone G44–26) and monoclonal mouse anti-CD209 (Clone DCN46) were obtained from BD Biosciences. .. Recombinant human Gal-9 protein (Cat. number 9064-GA) was obtained from R&D systems. .. SARS-CoV-2 Spike Protein (RBD) Chimeric Recombinant Rabbit Monoclonal Antibody (P05DHuRb) tagged with Alexa Fluor 647 was obtained from eBioscience.

    Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia
    Article Snippet: In the primary samples, CD34 + cells were enriched from mononuclear cells using the Indirect CD34 MicroBead Kit (Miltenyi Biotec) and preincubated in RPMI-1640 with 10% fetal calf serum (FCS) for 2 hours at 37°C. .. Preincubated primary samples and cell lines were washed using prewarmed phosphate-buffered saline and subsequently incubated in RPMI-1640 without FCS and stimulated by 10 ng/mL recombinant human Gal-9 protein (R&D Systems). .. For the assays with inhibitors, cells were incubated in the presence of A-419259 (Cayman Chemical), U0126 (Promega), LY294002 (Abcam), human dickkopf Wnt signaling pathway inhibitor 1 (DKK-1) recombinant protein (Peprotech), and a Src family inhibitors set including protein phosphatase 1 (PP1), PP2, and aminogenistein (AMGT) (proteinkinase.de) for 2 hours before stimulation with Gal-9.

    Article Title: Discovery of Small-Molecule TIM-3 Inhibitors for Acute Myeloid Leukemia Using Pharmacophore-Based Virtual Screening.
    Article Snippet: T-cell immunoglobulin and mucin domain 3 (TIM-3) is a negative immune checkpoint that represents a promising target for cancer immunotherapy.. Although encouraging results have been observed for TIM-3 inhibition in the context of acute myeloid leukemia (AML), targeting TIM-3 is currently restricted to monoclonal antibodies (mAbs).. To fill this gap, we implemented a pharmacophore-based screening approach to identify small-molecule TIM-3 inhibitors.

    Article Title: Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody
    Article Snippet: .. Inhibition of the Gal-9/TIM-3 interaction was assayed using recombinant human Gal-9 protein (R&D Systems, 2045-GA) coated at 2 μg/mL onto ELISA assay plates and then blocked with 3% BSA (Sigma, A-3912). .. Recombinant Human TIM-3-Fc Chimera protein (R&D Systems, 2365-TM), which contains full ECD of TIM-3, was biotinylated with EZ-Link Sulfo-NHS-LCBiotinylation kit (Thermo Scientific, 21327).

    Article Title: Early steps of multi-receptor viral interactions dissected by high-density, multi-color single molecule mapping in living cells
    Article Snippet: Monoclonal mouse anti-human CD44 (Clone G44-26) and monoclonal mouse anti-CD209 (Clone DCN46) were obtained from BD Biosciences. .. Recombinant human Gal-9 protein (Cat. number 9064-GA) was obtained from R&D systems. .. SARS-CoV-2 Spike Protein (RBD) Chimeric Recombinant Rabbit Monoclonal Antibody (P05DHuRb) tagged with Alexa FluorTM 647 was obtained from eBioscience.

    Article Title: Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody
    Article Snippet: .. Inhibition of the Gal-9/TIM-3 interaction was assayed using recombinant human Gal-9 protein (R&D Systems, 2045-GA) coated at 2 μg/mL onto ELISA assay plates and then blocked with 3% BSA (Sigma, A-3912). .. Recombinant Human TIM-3-Fc Chimera protein (R&D Systems, 2365-TM), which contains full ECD of TIM-3, was biotinylated with EZ-Link Sulfo-NHS-LC-Biotinylation kit (Thermo Scientific, 21327).

    Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia.
    Article Snippet: Cell stimulation by Gal-9 and incubation with or without inhibitors In the primary samples, CD34+ cells were enriched from mononuclear cells using the Indirect CD34 MicroBead Kit (Miltenyi Biotec) and preincubated in RPMI-1640 with 10% fetal calf serum (FCS) for 2 hours at 37◦C. .. Preincubated primary samples and cell lines were washed using prewarmed phosphate-buffered saline and subsequently incubated in RPMI-1640 without FCS and stimulated by 10 ng/mL recombinant human Gal-9 protein (R&D Systems). .. For the assays with inhibitors, cells were incubated in the presence of A-419259 (Cayman Chemical), U0126 (Promega), LY294002 (Abcam), human dickkopf Wnt signaling pathway inhibitor 1 (DKK-1) recombinant protein (Peprotech), and a Src family inhibitors set including protein phosphatase 1 (PP1), PP2, and aminogenistein (AMGT) (proteinkinase.de) for 2 hours before stimulation with Gal-9.

    Article Title: Early Steps of Individual Multireceptor Viral Interactions Dissected by High-Density, Multicolor Quantum Dot Mapping in Living Cells.
    Article Snippet: Antibodies and Reagents.Monoclonal mouse antihuman CD44 (Clone G44−26) and monoclonal mouse anti-CD209 (Clone DCN46) were obtained from BD Biosciences. .. Recombinant human Gal-9 protein (Cat. number 9064-GA) was obtained from R&D systems. .. SARS-CoV-2 Spike Protein (RBD) Chimeric Recombinant Rabbit Monoclonal Antibody (P05DHuRb) tagged with Alexa Fluor 647 was obtained from eBioscience.

    Saline:

    Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia
    Article Snippet: In the primary samples, CD34 + cells were enriched from mononuclear cells using the Indirect CD34 MicroBead Kit (Miltenyi Biotec) and preincubated in RPMI-1640 with 10% fetal calf serum (FCS) for 2 hours at 37°C. .. Preincubated primary samples and cell lines were washed using prewarmed phosphate-buffered saline and subsequently incubated in RPMI-1640 without FCS and stimulated by 10 ng/mL recombinant human Gal-9 protein (R&D Systems). .. For the assays with inhibitors, cells were incubated in the presence of A-419259 (Cayman Chemical), U0126 (Promega), LY294002 (Abcam), human dickkopf Wnt signaling pathway inhibitor 1 (DKK-1) recombinant protein (Peprotech), and a Src family inhibitors set including protein phosphatase 1 (PP1), PP2, and aminogenistein (AMGT) (proteinkinase.de) for 2 hours before stimulation with Gal-9.

    Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia.
    Article Snippet: Cell stimulation by Gal-9 and incubation with or without inhibitors In the primary samples, CD34+ cells were enriched from mononuclear cells using the Indirect CD34 MicroBead Kit (Miltenyi Biotec) and preincubated in RPMI-1640 with 10% fetal calf serum (FCS) for 2 hours at 37◦C. .. Preincubated primary samples and cell lines were washed using prewarmed phosphate-buffered saline and subsequently incubated in RPMI-1640 without FCS and stimulated by 10 ng/mL recombinant human Gal-9 protein (R&D Systems). .. For the assays with inhibitors, cells were incubated in the presence of A-419259 (Cayman Chemical), U0126 (Promega), LY294002 (Abcam), human dickkopf Wnt signaling pathway inhibitor 1 (DKK-1) recombinant protein (Peprotech), and a Src family inhibitors set including protein phosphatase 1 (PP1), PP2, and aminogenistein (AMGT) (proteinkinase.de) for 2 hours before stimulation with Gal-9.

    Incubation:

    Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia
    Article Snippet: In the primary samples, CD34 + cells were enriched from mononuclear cells using the Indirect CD34 MicroBead Kit (Miltenyi Biotec) and preincubated in RPMI-1640 with 10% fetal calf serum (FCS) for 2 hours at 37°C. .. Preincubated primary samples and cell lines were washed using prewarmed phosphate-buffered saline and subsequently incubated in RPMI-1640 without FCS and stimulated by 10 ng/mL recombinant human Gal-9 protein (R&D Systems). .. For the assays with inhibitors, cells were incubated in the presence of A-419259 (Cayman Chemical), U0126 (Promega), LY294002 (Abcam), human dickkopf Wnt signaling pathway inhibitor 1 (DKK-1) recombinant protein (Peprotech), and a Src family inhibitors set including protein phosphatase 1 (PP1), PP2, and aminogenistein (AMGT) (proteinkinase.de) for 2 hours before stimulation with Gal-9.

    Article Title: TIM-3 signaling hijacks the canonical Wnt/β-catenin pathway to maintain cancer stemness in acute myeloid leukemia.
    Article Snippet: Cell stimulation by Gal-9 and incubation with or without inhibitors In the primary samples, CD34+ cells were enriched from mononuclear cells using the Indirect CD34 MicroBead Kit (Miltenyi Biotec) and preincubated in RPMI-1640 with 10% fetal calf serum (FCS) for 2 hours at 37◦C. .. Preincubated primary samples and cell lines were washed using prewarmed phosphate-buffered saline and subsequently incubated in RPMI-1640 without FCS and stimulated by 10 ng/mL recombinant human Gal-9 protein (R&D Systems). .. For the assays with inhibitors, cells were incubated in the presence of A-419259 (Cayman Chemical), U0126 (Promega), LY294002 (Abcam), human dickkopf Wnt signaling pathway inhibitor 1 (DKK-1) recombinant protein (Peprotech), and a Src family inhibitors set including protein phosphatase 1 (PP1), PP2, and aminogenistein (AMGT) (proteinkinase.de) for 2 hours before stimulation with Gal-9.

    Inhibition:

    Article Title: Discovery of Small-Molecule TIM-3 Inhibitors for Acute Myeloid Leukemia Using Pharmacophore-Based Virtual Screening.
    Article Snippet: T-cell immunoglobulin and mucin domain 3 (TIM-3) is a negative immune checkpoint that represents a promising target for cancer immunotherapy.. Although encouraging results have been observed for TIM-3 inhibition in the context of acute myeloid leukemia (AML), targeting TIM-3 is currently restricted to monoclonal antibodies (mAbs).. To fill this gap, we implemented a pharmacophore-based screening approach to identify small-molecule TIM-3 inhibitors.

    Article Title: Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody
    Article Snippet: .. Inhibition of the Gal-9/TIM-3 interaction was assayed using recombinant human Gal-9 protein (R&D Systems, 2045-GA) coated at 2 μg/mL onto ELISA assay plates and then blocked with 3% BSA (Sigma, A-3912). .. Recombinant Human TIM-3-Fc Chimera protein (R&D Systems, 2365-TM), which contains full ECD of TIM-3, was biotinylated with EZ-Link Sulfo-NHS-LCBiotinylation kit (Thermo Scientific, 21327).

    Article Title: Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody
    Article Snippet: .. Inhibition of the Gal-9/TIM-3 interaction was assayed using recombinant human Gal-9 protein (R&D Systems, 2045-GA) coated at 2 μg/mL onto ELISA assay plates and then blocked with 3% BSA (Sigma, A-3912). .. Recombinant Human TIM-3-Fc Chimera protein (R&D Systems, 2365-TM), which contains full ECD of TIM-3, was biotinylated with EZ-Link Sulfo-NHS-LC-Biotinylation kit (Thermo Scientific, 21327).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Discovery of Small-Molecule TIM-3 Inhibitors for Acute Myeloid Leukemia Using Pharmacophore-Based Virtual Screening.
    Article Snippet: T-cell immunoglobulin and mucin domain 3 (TIM-3) is a negative immune checkpoint that represents a promising target for cancer immunotherapy.. Although encouraging results have been observed for TIM-3 inhibition in the context of acute myeloid leukemia (AML), targeting TIM-3 is currently restricted to monoclonal antibodies (mAbs).. To fill this gap, we implemented a pharmacophore-based screening approach to identify small-molecule TIM-3 inhibitors.

    Article Title: Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody
    Article Snippet: .. Inhibition of the Gal-9/TIM-3 interaction was assayed using recombinant human Gal-9 protein (R&D Systems, 2045-GA) coated at 2 μg/mL onto ELISA assay plates and then blocked with 3% BSA (Sigma, A-3912). .. Recombinant Human TIM-3-Fc Chimera protein (R&D Systems, 2365-TM), which contains full ECD of TIM-3, was biotinylated with EZ-Link Sulfo-NHS-LCBiotinylation kit (Thermo Scientific, 21327).

    Article Title: Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody
    Article Snippet: .. Inhibition of the Gal-9/TIM-3 interaction was assayed using recombinant human Gal-9 protein (R&D Systems, 2045-GA) coated at 2 μg/mL onto ELISA assay plates and then blocked with 3% BSA (Sigma, A-3912). .. Recombinant Human TIM-3-Fc Chimera protein (R&D Systems, 2365-TM), which contains full ECD of TIM-3, was biotinylated with EZ-Link Sulfo-NHS-LC-Biotinylation kit (Thermo Scientific, 21327).



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